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The Pennsylvania Veterinary Medical Association (PVMA) is the only statewide professional membership association dedicated exclusively to the profession of veterinary medicine and the interests of the veterinary team. The focus of our association is professional development, advocacy, and practice vitality to ensure the continued success of our members.

 

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New Brucella Canis Tests Available At PADLS

Posted By Jaime Markle, Friday, June 1, 2018

Brucella canis bacteria

Photo credit: Wikimedia Commons 

New Brucella Canis Tests Available At PADLS

Authored by: David L Thompson MS, PhD, and Deepanker Tewari BVSc PhD DACVM

Canine brucellosis is an important, incurable reproductive disease of dogs that is caused by Brucella canis. Natural transmission of canine brucellosis can occur by several routes. B. canis organisms are present in abundance in aborted material and vaginal discharge. Shedding of B. canis may occur for up to six weeks after an abortion. Semen, seminal fluid and urine from infected males have also been shown to be sources of infection. The organism can be present in blood, milk, saliva, nasal and ocular secretions, and in the feces. Carrier animals shed the organisms intermittently and present a risk to other dogs and even for humans. The carrier animals must be identified and removed from the breeding population to stop the spread of disease.

Diagnosis of canine brucellosis is best accomplished by use of diagnostic aids such as serology, PCR and culture.  PADLS-Harrisburg now offers both serology and culture tests for B canis detection. The lab has recently expanded serology test offerings for detection of canine brucellosis.  The Rapid Slide Agglutination Test (RSAT), both with and without 2-mercaptoethanol (2-ME), will continue to be used as an initial screening tool.  While this test produces few false negative results, false positive results are common.  Previously, a serum sample yielding an “RSAT pos./RSAT-2ME neg.” result would prompt a request for a client to resubmit another serum sample after 4 weeks to distinguish an acute from a chronic infection. In an infected animal, this follow up testing conducted after 4 weeks normally will show the RSAT-2ME test to be positive following conversion of IgM to IgG antibodies against Brucella.  However, in a number of scenarios we examined, when a second sample was received, no change was seen in the original reactivity.  This result prompted us to introduce additional tests during primary screening in our testing algorithm to address false positives.  In addition to the RSAT -/+ screening test, clients can now request the B. canis indirect immunofluorescent antibody (IFA) test.  The IFA test, is a supplementary screening tool and can help resolve false positives seen with the RSAT test--particularly when RSAT is positive and the RSAT-2ME is negative.  Generally, an “RSAT pos./RSAT-2ME pos.” result indicates infection.  These samples were previously forwarded to the National Veterinary Services Laboratory for performance of the Tube Agglutination Test (TAT+2-ME) confirmation.  The TAT+2-ME test is a semi-quantitative test with increased specificity.  The TAT+2-ME test has now been validated by PVL and is offered as a follow up test.  This new test reduces both the turn-around time and the client shipping fee required for forwarding samples. The serological testing strategy used at the lab is summarized in the diagram (below). 

B canis serology algorithm at PADLS

The isolation of B. canis from a clinical specimen remains the diagnostic gold standard, but because of intermittent shedding, up to 3 consecutive cultures may be necessary to rule out the disease in suspect animals. Recently, PVL added culture option too to enhance the B. canis diagnostic test options. Clinicians should consider B. canis anytime reproductive problems occur whether chronic or acute and all dogs for breeding purposes should be screened for brucellosis.

For more details, visit PADLS.org.

 

Visit PADLS

 

PADLS-Pennsylvania Veterinary Laboratory is located at 2305 N Cameron St. Harrisburg, PA.

 

Tags:  antibodies  blood  breeding  brucella  canine  canis  carrier  cultures  diagnostic  disease  dogs  infection  organisms  padls  positives  reproductive  risk  sample  screening  serum  specimen  test  testing  urine 

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Veterinarians to Share the Love for Chester County Senior Citizens' Pets

Posted By Jaime Markle, Thursday, March 22, 2018
Updated: Wednesday, March 28, 2018

Veterinarians to Share the Love for Chester County Senior Citizen’s Pets

The Pennsylvania Veterinary Foundation (PVF), the charitable arm of PVMA, will be hosting a Share the Love event on Saturday, April 28, 2018, at the Phoenixville Area Senior Center located at 153 Church Street, Phoenixville, PA 19460. This veterinary care event is for dogs and cats of local senior citizens and will feature local veterinarians and technicians sharing the love of their profession and animals by donating their time to provide:

  • Basic exams,
  • Vaccinations,
  • Flea and tick treatment,
  • Microchipping, and
  • Nail trims.

This event coincides with World Veterinary Day, which is celebrated annually on the last Saturday in April. Share the Love will be from 10:00 am to 3:00 pm BY APPOINTMENT ONLY.

PVF began sharing the love for senior citizens in Chester County in 2016 when it created its Henry’s Helping Paws Fund. This pilot program began with a partnership with Meals on Wheels in Chester County. It provided free pet food to senior citizens’ pets, which allowed owners and their beloved pets to stay together. In an effort to expand the program to not only offer pet food, but to also provide veterinary care grants to the animals, PVF was able to secure a grant from PetSmart Charities®. This grant made the Share the Love event possible, along with The Last Chance (TLC) Fund, PVF’s grant program that helps to pay for veterinary care for animals in need.

Who qualifies for a free veterinary exam?

Seniors, age 62 and over, who are on a fixed or low income and are located in Chester County, PA are eligible for this event. Seniors who would like to take advantage of these free services are encouraged to call PVF at 888.550.7862 to make an appointment for their pet or click here. This is an appointment-only event, and unfortunately, cannot accommodate walk-ins. This event will provide basic care for pets. PVF will provide a care voucher up to a certain amount that can be redeemed at a participating veterinary practice in Chester County. 

How can PVMA members you help? Sign up to volunteer that day!

We are looking for veterinarians, CVTs, veterinary assistants and CVPMs to help us at the event. To sign up as a volunteer, click here.

Volunteer

 

 

Tags:  care  cat  cats  dog  dogs  exam  free  pets  PetSmart Charities  senior citizens  veterinary medicine  volunteer  wellness  World Veterinary Day 

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